From 3D Light to 3D Electron Microscopy
Shared experiences from the volume EM community
In this new 28-page Science/AAAS ebook, learn more about volume electron microscopy (vEM) and volume correlative light and electron microscopy (vCLEM) for life sciences.
Get an introduction to the community, read articles and watch videos about vEM and vCLEM workflows, and get inspired from a discussion among electron microscopy experts from both academia and industry.
Table of Contents
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Sharing the science behind 3D electron microscopy |
Sean Sanders |
Community under the microscope |
Alexandra Elli, Daniel Hartmann, and Bernhard Zimmermann |
Sample preparation for volume correlative light and electron microscopy |
Jean Daraspe, Olivia Muriel, Sophie G. Martin, Christel Genoud |
Breaking down the correlative workflow for array tomography: |
Ian J. White1, Steve Furzeland2 Jemima J. Burden1 |
X-ray targeting of Arabidopsis pollen tetrads for SBF-SEM |
Anna Kremer1, Kimberly Meechan2, Marianne Beckwith2, Saskia Lippens1 |
Cryo-correlative light and electron microscopy for cellular tomography |
Anna M. Steyer, Zhengyi Yang, Simone Mattei |
Fluorescence-based targeting of a plankton cell for FIB-SEM |
Karel Mocaer, Giulia Mizzon, Manuel Gunkel, Aliaksandr Halavatyi, Yannick Schwab, Paolo Ronchi |
Introducing high-throughput serial section acquisition for ZEISS MultiSEM |
Anna Lena Eberle, Tomasz Garbowski, Stephan Nickell |
Panel discussion: vEM/vCLEM community building |
Johanna Bischof (Euro-BioImaging Bio-Hub, EMBL, Germany), Lucy Collinson (The Francis Crick Institute, UK), Kedar Narayan (NIH-NCI, USA), Yannick Schwab (EMBL, Germany), Paul Verkade (University of Bristol, UK), Alexandra Elli (Carl Zeiss Microscopy, Germany), Rosy Manser (Carl Zeiss Microscopy, UK) |